Recent Cell-Based Assay Papers of Note (Dec. 2006 - Jan. 2007)
Journal Title Lead Institution(s) Synopsis
Analytical Chemistry
2006 Dec 15; 78(24): 8175-82
Cell-based indicator to visualize picomolar dynamics of nitric oxide release from living cells University of Tokyo, Japan Discusses a cell-based indicator for visualizing picomolar dynamics of nitric oxide release from living cells, and demonstrates its use in indicating the extent of diffusing NO from single vascular endothelial cells
Assay and Drug Development Technology
2006 Dec; 4(6): 679-688
A simple cell-based HTS assay system to screen for inhibitors of p53-Hdm2 protein-protein interactions Fisher BioImage, Soborg, Denmark Demonstrates the use of BioImage's p53-Hdm2 Redistribution high-content imaging assay in a pilot screen of 3,165 small-molecule compounds for inhibitors of the p53-Hdm2 interaction
Assay and Drug Development Technology
2006 Dec; 4(6): 689-94
A nonadherent cell-based HTS assay for N-type calcium channel using calcium 3 dye Johnson & Johnson, Spring House, Penn. Investigates the application of no-wash FLIPR calcium assay kits from Molecular Devices to assay N-type calcium channel activity using a non-adherent cell line in a high-throughput manner
Biochemical Pharmacology
2007 Jan 1; 73(1): 25-33
Pharmacological profiling of disulfiram using human tumor cell lines and human tumor cells from patients Uppsala University Hospital, Sweden Characterizes disulfarim in vitro with regard to tumor-type specificity, possible mechanisms of action, and drug resistance and cell death in human tumor cells and cell lines using the fluorimetric microculture cytotoxicity assay and Cellomics ArrayScan
Biomaterials
2007 Feb; 28(6): 1048-60
Combinatorial protein display for the cell-based screening of biomaterials that direct neural stem cell differentiation Institute for Frontier Medical Sciences, Kyoto University, Japan Describes the use of cell-based arrays to identify composite biomaterials that consist of extracellular matrix components and growth factors and that direct neural stem cell differentiation
Cytometry A
2007 Jan 24;
[Epub ahead of print]
A single platform image cytometer for resource-poor settings to monitor disease progression in HIV infection University of Twente, The Netherlands Describes an inexpensive imaging cytometer manufactured by Immunicon and demonstrates its ability to enumerate CD4(+) lymphocytes in blood samples from HIV patients
Journal of Biomolecular Screening
2007 Jan 4;
[Epub ahead of print]
Image-based screening for the identification of novel proteasome inhibitors Uppsala University Hospital, Sweden Describes an image-based screening assay using HEK 293 ZsGreen Proteasome Sensor cell lines and the Cellomics ArrayScan for the identification of compounds with proteasome-inhibiting activity
Journal of Biomolecular Screening
2006 Dec 14;
[Epub ahead of print]
A high-throughput cell-based assay to identify specific inhibitors of transcription factor AP-1 National Cancer Institute, Frederick, Md. Describes the development of a 384-well cellular Förster resonance energy transfer assay to screen synthetic and natural product libraries for non-cytotoxic inhibitors of mitogen-activated AP-1 activity
Journal of Biomolecular Screening
2006 Nov 27;
[Epub ahead of print]
Microplate orbital mixing improves high-throughput cell-based reporter assay readouts Genomics Institute of the Novartis Foundation, San Diego, Calif.; and Invitrogen, Madison, Wisc. Demonstrates that incorporating a microplate orbital mixing step into 96- and 384-well microplate cell-based luciferase reporter assays can greatly improve reporter readouts
Journal of Lipid Research
2007 Jan 9;
[Epub ahead of print]
Cell-based multiwell assays for detection of substrate accumulation and oxidation AstraZeneca, Mölndal, Sweden Describes multiwell assays based on the scintillation proximity assay for detecting accumulation and subsequent oxidation of 14C-labeled substrates in cultured cells
Journal of Pharmaceutical and Biomedical Analysis
2007 Jan 17; 43(2): 666-76
Development and validation of a quantitative cell-based bioassay for comparing the pharmacokinetic profiles of two recombinant erythropoietic proteins in serum Amgen, Thousand Oaks, Calif. Validates an in vitro cell-based assay to assess the pharmacokinetic profiles of two novel therapeutic recombinant proteins with erythropoiesis-stimulating properties in Sprague-Dawley rats
Life Sciences
2006 Dec 3; 80(1): 17-23
Monitoring ligand-mediated internalization of G protein-coupled receptor as a novel pharmacological approach Kyoto University, Japan Examines whether visualizing the ligand-mediated internalization of a GPCR using GFP-labeling and fluorescence imaging can be exploited for pharmacological evaluations
Nucleic Acids Research
2007 Jan; 35 (Database issue): D492-7
GenomeRNAi: a database for cell-based RNAi phenotypes German Cancer Research Institute, Heidelberg, Germany Introduces the GenomeRNAi database, which provides access to published RNAi phenotypes obtained from cell-based screens in C. elegans and Drosophila, and maps them to their genomic locus, including non-specific regions
PLoS One
2007 Jan 17; 2:e163
Toxicity assays in nanodrops combining bioassay and morphometric endpoints Commissariat a l'Energie Atomique Centre de Grenoble, Grenoble, France Describes a new in vitro method for toxicity testing, combining cell-based assays in nanodrop Cell-on-Chip format with the use of a genetically engineered stress-sensitive hepatic cell line
Prostate
2007 Jan 11;
[Epub ahead of print]
Rapid, non-destructive, cell-based screening assays for agents that modulate growth, death, and androgen receptor activation in prostate cancer cells University of British Columbia, Vancouver, Canada Describes the development of a non-invasive, cell-based screening assay to assess factors that modulate prostate cancer growth in LNCaP cells expressing enhanced green fluorescent protein