Recent
Cell-Based Assay Papers of Note (Dec. 2006 - Jan. 2007)
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Journal | Title | Lead Institution(s) | Synopsis |
Analytical Chemistry
2006 Dec 15; 78(24): 8175-82 |
Cell-based indicator to visualize picomolar dynamics of nitric oxide release from living cells | University of Tokyo, Japan | Discusses a cell-based indicator for visualizing picomolar dynamics of nitric oxide release from living cells, and demonstrates its use in indicating the extent of diffusing NO from single vascular endothelial cells |
Assay and Drug
Development Technology 2006 Dec; 4(6): 679-688 |
A simple cell-based HTS assay system to screen for inhibitors of p53-Hdm2 protein-protein interactions | Fisher BioImage, Soborg, Denmark | Demonstrates the use of BioImage's p53-Hdm2 Redistribution high-content imaging assay in a pilot screen of 3,165 small-molecule compounds for inhibitors of the p53-Hdm2 interaction |
Assay and Drug
Development Technology 2006 Dec; 4(6): 689-94 |
A nonadherent cell-based HTS assay for N-type calcium channel using calcium 3 dye | Johnson & Johnson, Spring House, Penn. | Investigates the application of no-wash FLIPR calcium assay kits from Molecular Devices to assay N-type calcium channel activity using a non-adherent cell line in a high-throughput manner |
Biochemical Pharmacology
2007 Jan 1; 73(1): 25-33 |
Pharmacological profiling of disulfiram using human tumor cell lines and human tumor cells from patients | Uppsala University Hospital, Sweden | Characterizes disulfarim in vitro with regard to tumor-type specificity, possible mechanisms of action, and drug resistance and cell death in human tumor cells and cell lines using the fluorimetric microculture cytotoxicity assay and Cellomics ArrayScan |
Biomaterials
2007 Feb; 28(6): 1048-60 |
Combinatorial protein display for the cell-based screening of biomaterials that direct neural stem cell differentiation | Institute for Frontier Medical Sciences, Kyoto University, Japan | Describes the use of cell-based arrays to identify composite biomaterials that consist of extracellular matrix components and growth factors and that direct neural stem cell differentiation |
Cytometry A 2007 Jan 24; [Epub ahead of print] |
A single platform image cytometer for resource-poor settings to monitor disease progression in HIV infection | University of Twente, The Netherlands | Describes an inexpensive imaging cytometer manufactured by Immunicon and demonstrates its ability to enumerate CD4(+) lymphocytes in blood samples from HIV patients |
Journal of Biomolecular
Screening 2007 Jan 4; [Epub ahead of print] |
Image-based screening for the identification of novel proteasome inhibitors | Uppsala University Hospital, Sweden | Describes an image-based screening assay using HEK 293 ZsGreen Proteasome Sensor cell lines and the Cellomics ArrayScan for the identification of compounds with proteasome-inhibiting activity |
Journal of Biomolecular
Screening 2006 Dec 14; [Epub ahead of print] |
A high-throughput cell-based assay to identify specific inhibitors of transcription factor AP-1 | National Cancer Institute, Frederick, Md. | Describes the development of a 384-well cellular Förster resonance energy transfer assay to screen synthetic and natural product libraries for non-cytotoxic inhibitors of mitogen-activated AP-1 activity |
Journal of Biomolecular
Screening 2006 Nov 27; [Epub ahead of print] |
Microplate orbital mixing improves high-throughput cell-based reporter assay readouts | Genomics Institute of the Novartis Foundation, San Diego, Calif.; and Invitrogen, Madison, Wisc. | Demonstrates that incorporating a microplate orbital mixing step into 96- and 384-well microplate cell-based luciferase reporter assays can greatly improve reporter readouts |
Journal of Lipid
Research 2007 Jan 9; [Epub ahead of print] |
Cell-based multiwell assays for detection of substrate accumulation and oxidation | AstraZeneca, Mölndal, Sweden | Describes multiwell assays based on the scintillation proximity assay for detecting accumulation and subsequent oxidation of 14C-labeled substrates in cultured cells |
Journal of Pharmaceutical
and Biomedical Analysis 2007 Jan 17; 43(2): 666-76 |
Development and validation of a quantitative cell-based bioassay for comparing the pharmacokinetic profiles of two recombinant erythropoietic proteins in serum | Amgen, Thousand Oaks, Calif. | Validates an in vitro cell-based assay to assess the pharmacokinetic profiles of two novel therapeutic recombinant proteins with erythropoiesis-stimulating properties in Sprague-Dawley rats |
Life Sciences
2006 Dec 3; 80(1): 17-23 |
Monitoring ligand-mediated internalization of G protein-coupled receptor as a novel pharmacological approach | Kyoto University, Japan | Examines whether visualizing the ligand-mediated internalization of a GPCR using GFP-labeling and fluorescence imaging can be exploited for pharmacological evaluations |
Nucleic Acids
Research 2007 Jan; 35 (Database issue): D492-7 |
GenomeRNAi: a database for cell-based RNAi phenotypes | German Cancer Research Institute, Heidelberg, Germany | Introduces the GenomeRNAi database, which provides access to published RNAi phenotypes obtained from cell-based screens in C. elegans and Drosophila, and maps them to their genomic locus, including non-specific regions |
PLoS One 2007 Jan 17; 2:e163 |
Toxicity assays in nanodrops combining bioassay and morphometric endpoints | Commissariat a l'Energie Atomique Centre de Grenoble, Grenoble, France | Describes a new in vitro method for toxicity testing, combining cell-based assays in nanodrop Cell-on-Chip format with the use of a genetically engineered stress-sensitive hepatic cell line |
Prostate 2007 Jan 11; [Epub ahead of print] |
Rapid, non-destructive, cell-based screening assays for agents that modulate growth, death, and androgen receptor activation in prostate cancer cells | University of British Columbia, Vancouver, Canada | Describes the development of a non-invasive, cell-based screening assay to assess factors that modulate prostate cancer growth in LNCaP cells expressing enhanced green fluorescent protein |