Recent
Cell-Based Assay Papers of Note
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Journal | Title | Lead Institution(s) | Synopsis |
Analytical Chemistry
2006 Nov 15; 78(22): 7625-7631 |
Electrochemical monitoring of cellular signal transduction with a secreted alkaline phosphatase reporter system | Tohoku University, Sendai, Japan | Demonstrates the electrochemical monitoring of signal transduction in 3-D cell culture using cellular microarrays and a secreted alkaline phosphatase reporter system |
Assay and Drug
Development Technologies 2006; 4(5): 545-553 |
Dynamic detection of natural killer cell-mediated cytotoxicity and cell adhesion by electrical impedance measurements | Novo Nordisk, Malov, Denmark | Demonstrates the use of the Acea real-time cell electrical sensing platform for detecting the toxic effects of natural killer cells |
Biomedical Microdevices
2006 Nov 11; [Epub ahead of print] |
A microfluidic platform for 3-dimensional cell culture and cell-based assays | Korea Advanced Institute of Science and Technology, Daejeon | Reports on a microfluidic platform that uses a peptide hydrogel to make a biocompatible microenvironment for cell culture and in situ cell-based assays |
Biomedical Microdevices
2006 Dec; 8(4): 361-74 |
Microplate cell-retaining methodology for high-content analysis of individual non-adherent unanchored cells in a population | Bar-Ilan University, Ramat Gan, Israel | Describes the development of a high-throughput microtiter plate cell retainer for high-content, time-dependent analysis of single non-adherent cells in a large cell population |
Biotechnology
and Applied Biochemistry 2006 Nov 8; [Epub ahead of print] |
A cell-based assay system for high-throughput screening of anti-wrinkle agents in human dermal fibroblast transfectant cells | Biospectrum Life Science Institute, Gyunggi Do, South Korea | Describes the development of a cell-based assay system for monitoring COL1A2 promoter activity to determine the influence of the activated promoter on human dermal fibroblasts |
Journal of Biomedical
Optics 2006 Sept/Oct; 11(5): 054024 |
Time-domain fluorescent plate reader for cell-based protein-protein interaction and protein conformation assays | Harvard Medical School/Massachusetts General Hospital, Charlestown, Mass. | Describes the use of a modified commercial fluorescence lifetime plate reader to develop a FRET-based cellular assay for protein-protein interactions and protein conformational changes |
Journal of Biomolecular
Screening 2006 Nov 12; [Epub ahead of print] |
A nonradioactive high-throughput/high-content assay for measurement of the human serotonin reuptake transporter function in vitro | DSM Nutritional Products, Kaiseraugst, Switzerland | Discusses the development of a microplate-based high-throughput screen for hSERT function that can be read in high-content modes using a sub-cellular imaging device |
Journal of Neuroscience
Methods 2006 Oct 16; [Epub ahead of print] |
Simultaneous monitoring of three key neuronal functions in primary neuronal cultures | University of Edinburgh, UK | Discusses the development of an assay that simultaneously monitors synaptic vesicle recycling and changes in intracellular free calcium ions in cultured neurons using FM4-64 and fura-2AM reporter dyes |
Journal
of Pharmacological and Toxicological Methods 2006 Nov-Dec; 54(3): 313-9 |
Cell-based assays for profiling activity and safety properties of cancer drugs | Berlex Biosciences, Richmond, Calif. | Describes the use of a panel of assays based on various normal and cancer cell types from human origin to evaluate select marketed anti-cancer drugs |
Journal of Toxicological
Sciences 2006 Oct; 31(4): 315-24 |
Physicochemical and cell-based approach for early screening of phospholipidosis-inducing potential | Pfizer Global R&D, Nagoya, Japan | Study examines the predictability of several physiochemical methods, including cell-based assays, for assessing phospholipidosis |
Methods
2006 Nov; 40(3): 287-93 |
Chemical genetic strategies to delineate MAP kinase signaling pathways using protein-fragment complementation assays | University of Montreal, Canada | Describes a strategy for directly analyzing MAP kinase signaling pathways in living cells using protein-fragment complementation assays based on intensely fluorescent proteins |
Molecular Biology
of the Cell 2006 Nov 8; [Epub ahead of print] |
High-content microscopy identifies new neurite outgrowth regulators | German Cancer Research Center, Heidelberg, Germany | Describes the establishment and application of a microscopy-based method for identifying novel proteins involved in neurite initiation and elongation |
Molecules and
Cells 2006 Oct 31; 22(2): 198-202 |
Establishment of an assay for P2X7 receptor-mediated cell death | Gwangju Institute of Science and Technology, Korea | Discusses the development of a cell-based assay for human P2X7 receptor antagonists that uses hP2X7-expressing HEK 293 cells |