Recent Cell-Based Assay Papers of Note
Journal Title Lead Institution(s) Synopsis
Analytical Chemistry
2006 Nov 15; 78(22): 7625-7631
Electrochemical monitoring of cellular signal transduction with a secreted alkaline phosphatase reporter system Tohoku University, Sendai, Japan Demonstrates the electrochemical monitoring of signal transduction in 3-D cell culture using cellular microarrays and a secreted alkaline phosphatase reporter system
Assay and Drug Development Technologies
2006; 4(5): 545-553
Dynamic detection of natural killer cell-mediated cytotoxicity and cell adhesion by electrical impedance measurements Novo Nordisk, Malov, Denmark Demonstrates the use of the Acea real-time cell electrical sensing platform for detecting the toxic effects of natural killer cells
Biomedical Microdevices
2006 Nov 11; [Epub ahead of print]
A microfluidic platform for 3-dimensional cell culture and cell-based assays Korea Advanced Institute of Science and Technology, Daejeon Reports on a microfluidic platform that uses a peptide hydrogel to make a biocompatible microenvironment for cell culture and in situ cell-based assays
Biomedical Microdevices
2006 Dec; 8(4): 361-74
Microplate cell-retaining methodology for high-content analysis of individual non-adherent unanchored cells in a population Bar-Ilan University, Ramat Gan, Israel Describes the development of a high-throughput microtiter plate cell retainer for high-content, time-dependent analysis of single non-adherent cells in a large cell population
Biotechnology and Applied Biochemistry
2006 Nov 8; [Epub ahead of print]
A cell-based assay system for high-throughput screening of anti-wrinkle agents in human dermal fibroblast transfectant cells Biospectrum Life Science Institute, Gyunggi Do, South Korea Describes the development of a cell-based assay system for monitoring COL1A2 promoter activity to determine the influence of the activated promoter on human dermal fibroblasts
Journal of Biomedical Optics
2006 Sept/Oct; 11(5): 054024
Time-domain fluorescent plate reader for cell-based protein-protein interaction and protein conformation assays Harvard Medical School/Massachusetts General Hospital, Charlestown, Mass. Describes the use of a modified commercial fluorescence lifetime plate reader to develop a FRET-based cellular assay for protein-protein interactions and protein conformational changes
Journal of Biomolecular Screening
2006 Nov 12; [Epub ahead of print]
A nonradioactive high-throughput/high-content assay for measurement of the human serotonin reuptake transporter function in vitro DSM Nutritional Products, Kaiseraugst, Switzerland Discusses the development of a microplate-based high-throughput screen for hSERT function that can be read in high-content modes using a sub-cellular imaging device
Journal of Neuroscience Methods
2006 Oct 16; [Epub ahead of print]
Simultaneous monitoring of three key neuronal functions in primary neuronal cultures University of Edinburgh, UK Discusses the development of an assay that simultaneously monitors synaptic vesicle recycling and changes in intracellular free calcium ions in cultured neurons using FM4-64 and fura-2AM reporter dyes
Journal of Pharmacological and Toxicological Methods
2006 Nov-Dec; 54(3): 313-9
Cell-based assays for profiling activity and safety properties of cancer drugs Berlex Biosciences, Richmond, Calif. Describes the use of a panel of assays based on various normal and cancer cell types from human origin to evaluate select marketed anti-cancer drugs
Journal of Toxicological Sciences
2006 Oct; 31(4): 315-24
Physicochemical and cell-based approach for early screening of phospholipidosis-inducing potential Pfizer Global R&D, Nagoya, Japan Study examines the predictability of several physiochemical methods, including cell-based assays, for assessing phospholipidosis
2006 Nov; 40(3): 287-93
Chemical genetic strategies to delineate MAP kinase signaling pathways using protein-fragment complementation assays University of Montreal, Canada Describes a strategy for directly analyzing MAP kinase signaling pathways in living cells using protein-fragment complementation assays based on intensely fluorescent proteins
Molecular Biology of the Cell
2006 Nov 8; [Epub ahead of print]
High-content microscopy identifies new neurite outgrowth regulators German Cancer Research Center, Heidelberg, Germany Describes the establishment and application of a microscopy-based method for identifying novel proteins involved in neurite initiation and elongation
Molecules and Cells
2006 Oct 31; 22(2): 198-202
Establishment of an assay for P2X7 receptor-mediated cell death Gwangju Institute of Science and Technology, Korea Discusses the development of a cell-based assay for human P2X7 receptor antagonists that uses hP2X7-expressing HEK 293 cells