Recent Cell-Based Assay Papers of Note
Journal	Title	Lead Institution(s)	Synopsis
Assay and Drug Development Technologies 2006 Aug; 4(4): 421-42	Quantitative characterization of mitosis-blocked tetraploid cells using high-content analysis	Cellomics, Pittsburgh, Pa.	Demonstrates the use of high-content screening to assay fluorescently labeled nocodazole-treated cells to verify that mitosis blocks results in tetraploid cells
Assay and Drug Development Technologies 2006 Aug; 4(4): 397-409	High-content screening analysis of the p38 pathway: Profiling of structurally related p38 alpha kinase inhibitors using cell-based assays	Amgen, Thousand Oaks, Calif.	Describes a profiling and potency comparison of a large set of p38alpha inhibitors with a set of cellular imaging assays in SW1353 chondrocytes and baby hamster kidney cells
Assay and Drug Development Technologies 2006 Aug; 4(4): 473-82	Cell-based assay strategy for identification of motif-specific RANK signaling pathway inhibitors	Bristol-Myers Squibb, Wallingford, Conn.	Describes a strategy for constructing cell-based systems for identifying compounds that inhibit signaling from two recently identified receptor activator of NF-κB (RANK) motifs
Assay and Drug Development Technologies 2006 Aug; 4(4): 461-71	Real-time and high-throughput monitoring of cAMP in live cells using a fluorescent membrane potential-sensitive dye	AnaSpec, San Jose, Calif.	Reports a live-cell cAMP assay to record real-time changes in intracellular cAMP by employing a mutated cyclic-nucleotide-gated ion channel as a cAMP biosensor and a new fluorescent membrane potential-sensitive dye
Assay and Drug Development Technologies 2006 Aug; 4(4): 451-60	A cellular assay for measuring the inhibition of glycogen synthase kinase-3 via the accumulation of beta-catenin in Chinese hamster ovary clone K1 cells	Serono Pharmaceutical Research Institute, Plan-les-Ouates, Switzerland	Describes the development of a cell-based assay designed to measure the activity of glycogen synthase kinase-3 via cellular beta-catenin accumulation using an antibody staining protocol and luminometric readout
Assay and Drug Development Technologies 2006 Aug; 4(4): 387-396	High-throughput screening identified a substituted imidazole as a novel RANK pathway-selective osteoclastogenesis inhibitor	Bristol-Myers Squibb, Wallingford, Conn.	Describes the use of a high-throughput cell-based assay using RAW264.7 cells stably transfected with a TRAP reporter gene to identify compounds that block osteoclastogenesis
Biochemical and Biophysical Research Communications 2006 Oct 13; 349(1): 186-191	A chemiluminescence-based reporter system to monitor nonsense-mediated mRNA decay	University of Heidelberg and European Molecular Biology Laboratory, Germany	Details a cell-based chemiluminescence reporter system that recapitulates the hallmark features of mammalian nonsense-mediated mRNA decay and may be useful for small-molecule screening
Drug Discovery Today 2006 Sept; 11(17-18): 785-91	Cytomics as a new potential for drug discovery	Max Planck Institute for Biochemistry, Martinsried, Germany	Review article discusses how high-content screening by image analysis or flow cytometry of clinical cell or tissue samples can, through reverse engineering of molecular profiles, detect new drug targets and support predictive medicine
Journal of Biomolecular Screening 2006 Aug; 11(5): 511-8	A novel method for analyzing [Ca2+] flux kinetics in high-throughput screening	Pfizer, Sandwich, UK	Describes a mathematical model to describe the time traces of cellular calcium fluxes mediated by the activation of GPCRs, based on measurements taken from a fluorometric imaging plate reader
Journal of Biomolecular Screening 2006 Aug; 11(5): 488-96	Population patch clamp improves data consistency and success rates in the measurement of ionic currents	Molecular Devices, Union City, Calif.	Describes a population patch clamp method in which a single voltage-clamp amplifier sums the whole-cell currents from multiple cells simultaneously, resulting in increased data consistency
Journal of Biomolecular Screening 2006 Aug 23; [Epub ahead of print]	Two approaches to drug discovery in SOD1-mediated ALS	Massachusetts General Hospital, Charlestown	Describes the design of two cell-based screening assays to identify small, brain-permeant molecules that inactivate expression of the SOD1 gene or increase the degradation of the SOD1 protein and may be useful in treating familial amyotrophic lateral sclerosis
Nature 2006 Jul 27; 442(7101): 403-11	Cells on chips	Massachusetts Institute of Technology, Cambridge, Mass.	Review article discusses highly integrated microdevices that mimic the complex biochemistry and geometry of the extracellular matrix for use in biomedical and pharmaceutical research, and as point-of-care devices in clinical settings
Proceedings of the National Academy of Sciences 2006 Aug 8; 103(32): 11880-5	A genome-wide screen for components of the RNAi pathway in Drosophila cultured cells	Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, Md.	Describes the use of luciferase reporter constructs and a cultured cell-based assay to perform a genome-wide screen for components of the RNAi pathway in Drosophila melanogaster
Virology Journal 2006 Sep 1; 3(1): 68 [Epub ahead of print]	Natural products that reduce rotavirus infectivity identified by a cell-based moderate-throughput screening assay	Montana State University, Bozeman	Details the use of a cell-based, moderate-throughput screening assay to screen 280 compounds for those that reduce rotavirus infectivity in vitro